ABSTRACT
Objective: To ascertain the condition for extracting tetramethylpyrazine from Rhizoma Chuanxiong with alcohol. Methods: With the transferring rate of tetramethylpyrazine as index, the optimum extraction condition for the first time was selected by uniform test, and the times of extraction was studied. Results: Taking 80% alcohol as solvent, Rhizoma Chuanxiong was extracted two times with 8 and 6 times volume of alcohol for 2hr and 1.5hr, respectively. In this condition, tetramethylpyrazine could be effectively extracted. Conclusion: The experimental results provided the basis for the ascertainment of extraction process of Rhizoma Chuanxiong preparation.
ABSTRACT
Objective: To ascertain the factors and levels in the orthogonal design by drawing the extraction amount time curve of total alkaloid of Rhizoma Coptidis in different solvents. Methods:The extraction amount time curve of total alkaloid was plotted with the extraction amount of total alkaloid as a marker. The orthogonal design was used to select the optimum extraction process according to the contents of total alkaloid and berberine, and extract yield. Results:The extraction amound of total alkaloid related to volume and kinds of solvent and extraction time. The optimum exttraction process was as follows: adding 50% alcohol into meterials and refluxing and extracting for 3 times, 120 min every time. Conclusion:The investigation on the relation between total alkaloid extraction amount and time is of an instructive significance in orthogonal design. The optimum extraction process of Rhizoma Coptidis will provide an experimental basis for industrial production.